Paediatric Lumber Puncture
Paediatric Lumber Puncture
Indications in ED
- As part of the septic screen of a neonate
- As part of the septic screen of an infant or child where there are concerns for meningitis
Note- there are many other indications for paediatric lumbar punctures, but these are often performed on the ward by inpatient teams or after neuroimaging.
Contraindications
- Reduced GCS
- Signs of raised ICP, such as: altered pupil responses, bulging fontanelle, posturing.
- New focal neurological signs, such as: hemiparesis, ocular palsies
- Local infection at the needle insertion site
- Known coagulation defects or thrombocytopaenia
- Cardiovascular compromise/shock
- Respiratory compromise, such as apnoeas.
Equipment
- Dressing trolley
- Sterile: gown, gloves, mask, hat, hand towel, drape
- Large dressing pack
- Skin antiseptic
- o Infants, children, adolescents: chlorhexidine 2% & isopropyl alcohol 70% (e.g. Maxi swabstick) or
o Povidone iodine 10% if known sensitivity to chlorhexidine
o Neonates ≥ 28 weeks gestation: chlorhexidine 1% & isopropyl alcohol 70% (swab stick preferable)
- Spinal lumber puncture needle- 22G or 25G bevelled needles with stylet (the use of needles without a stylet has an associated rare risk of spinal epidermoid tumours)
o Pencil point is preferred in older children to reduce risk of headache (evidence is not convincing in younger children)
- Topical anaesthetic cream
- Local anaesthetic with syringe and needle
- Sucrose
- Manometer set
- 3 sterile CSF specimen containers
- Small transparent occlusive dressing

Prior to procedure
- Apply local anaesthetic cream to the back if time allows
- Ensure child is on continuous pulse oximetry/HR monitoring and consider cardiac monitoring where appropriate
- Ensure enough staff present for procedure. Often need
o Proceduralist
o Assistant to pass specimen tubes and to monitor observations
o Assistant to hold the baby
o May even need a 3rd assistant if sedation (such as sucrose) required
Procedure
- Perform hand hygiene
- Open sterile equipment onto sterile field trolley
- Assistant to position the patient
o Position the patient in a lateral position with the patient facing the positioning nurse
o Patient knees and chin are to be drawn to the chest, and body well flexed (foetal position)
o The hips should be vertical to align the iliac crests i.e. back should be 90 degrees to the bed
o The patients back should be positioned parallel and close to the edge of the bed.
- Identify the LP site
o A line between the top of the iliac crest intersects the spine at approximately the L3/L4 interspace:
o Site for needle insertion should be L3/L4 or L4/L5 interspace.
- Apply mask. Perform aseptic hand wash and apply gown and gloves
- Clean skin with antiseptic swab sticks
o Wipe antiseptic swab in a circular motion commencing at the proposed insertion site
o Repeat with second swab stick or sterile forceps and gauze
- Place drape onto patient
- Palpate the needle insertion point
- If using local anaesthetic then infiltrate this into the site and allow 1-2 minutes for it to work
- Ensure the skin is dry prior to needle insertion
- Perform the LP
o Hold the spinal needle so that bevel is in the superior position (facing up)
o With the stylet in position, insert the needle through the skin and wait for any patient movement to stop
o Aiming for the umbilicus, advance the needle in the direction towards patient’s umbilicus until there is a decrease in resistance.
o Remove the stylet and check for CSF appearing at the needle hub:
- If CSF is not flowing: Replace stylet fully and advance or reposition the needle slightly and recheck for CSF as above.
- If CSF is flowing
- If opening pressure measurement is required, connect manometer to spinal needle.
- Collect 10 drops in each (x3) sterile container
o Pass them to the assistant who can label them 1-3 in order of collection and label them with the patient details
- When sample collection is complete, reinsert the stylet, then remove the needle and stylet as one
- Use sterile gauze to apply gentle pressure to the insertion site
- Cover the insertion site with a transparent occlusive dressing



Post Procedure
- A minimum of hourly neurological observations for 4 hours for all patients post procedure (may require longer depending on underlying illness).
- Patients should be allowed to mobilise as soon as it is safe to do so and, if applicable, on recovering from sedating medication.
Complications
- Headaches (common, up to 15%)
- Local back pain
- Transient / persistent paraesthesia / numbness
- Cerebral herniation ( bradycardia, apnoeas, oxygen desaturations)
- Subdural haemorrhage
- Spinal epidural haemorrhage
- Paraplegia
- Infection (local and introduction into CSF spaces)
- Cardiorespiratory compromise due to positioning
- Spinal epidermoid tumours associated with needles without a stylet (rare)
